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total smad2 3  (Cell Signaling Technology Inc)


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    Structured Review

    Cell Signaling Technology Inc total smad2 3
    Total Smad2 3, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 4485 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/total+smad2+3/Smad2%2F3+Antibody/pm41828479-238-72-75
    Average 96 stars, based on 4485 article reviews
    total smad2 3 - by Bioz Stars, 2026-09
    96/100 stars

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    other:

    Article Title: The Nephroprotective Effects of Hibiscus sabdariffa Leaf and Ellagic Acid in Vitro and in Vivo Models of Hyperuricemic Nephropathy.
    Article Snippet: Hyperuricemic nephropathy (HN) is caused by urate crystals that get deposited in the kidney and contribute to renal fibrosis.. Uric acid (UA) has been proven to directly cause renal mesangial cell oxidative stress and fibrosis in the pathogenesis of HN.. Some antioxidants can be used as chemopreventive agents of HN.

    Article Title: Type I interferons and TGF-β cooperate to induce liver fibrosis during HIV-1
    Article Snippet: Protein 69 extracts were resolved on SDS-PAGE (12%), transferred onto a nitrocellulose membrane 70 and incubated with the following primary antibodies at 1:250 to 1:1000 dilution: phospho-71 SMAD2/3 (#8828) , phospho-STAT1 Tyr701 (#9167), phospho-STAT1 Ser727 (#8826), 72 phospho-p38 (#4511) and phospho-ERK1/2 (#9101) and total SMAD2/3 (#8685), STAT1 73 (#14994), p38 (#9212), ERK1/2 (#4695), all from Cell Signal; α-SMA (1:100, ab7817), 74 CCND1 (#ab16663), CCNA2 (#ab181591) from Abcam and β-actin (#A3854, 75 Sigma/Millipore); secondary antibodies were either anti-mouse or anti-rabbit coupled with 76 horseradish peroxidase, and bands were revealed using the ECL (Millipore and 77 Invitrogen) with BioRad CCD camera.

    Article Title: Adipocyte retinoic acid receptor α prevents obesity and steatohepatitis by regulating energy expenditure and lipogenesis.
    Article Snippet: Antibodies against acetyl-CoA carboxylase (ACC) (cat # 3662), phosphoAMP-activated protein kinase (pAMPK) (cat # 2535), AMP-activated protein kinase (AMPK) (cat # 2603), phospho-hormone–sensitive lipase (pHSL) (cat # 45804), hormone-sensitive lipase (HSL) (cat # 4107), adipose tissue triglyceride lipase (ATGL) (cat # 2138), uncoupling protein 1 (UCP1) (cat # 14670), phospho serine/threonine kinase 1 (pAKT) (cat # 4060), total AKT (cat # 9272), cleaved caspase 3 (Cat # 9661), total caspase 3 (Cat # 9662), phospho-Smad2/3 (Cat # 8828), or total Smad2/3 (Cat # 5678) were purchased from Cell Signaling Technology (Boston, MA).

    Article Title: High glucose induces an activated state of partial epithelial-mesenchymal transition in human primary tubular cell cultures.
    Article Snippet: To detect the specific protein bands, antibodies against Phospho-Ser 465/ 467 Smad2 (1:1000, Cell Signaling Technology), Total Smad2/3 (1:1000, Cell Signaling Technology), α-Tubulin (1:1000, Cell Signaling Technology) and Histone-H3 (1:4000, clone 96C10, Cell Signaling Technology) have been used. (B) PAI-1 transcript expression evaluated by Real-time PCR in primary tubular cell cultures at the indicated time points in control (ctrl) and high glucose (HG) conditions.

    Article Title: Nephroprotective effect of AT-MSCs against cisplatin-induced EMT is improved by azilsartan via attenuating oxidative stress and TGF-β/Smad signaling.
    Article Snippet: Equal protein extracts (50 μg) were separated on a 12.5% sodium dodecyl sulphate polyacrylamide gel electrophoresis and then transmitted to polyvinylideneflouride membranes which were blocked at room temperature for 1 h with 5% (w/v) non-fat dry milk and then incubated at 4 ◦C overnight with primary antibodies for E-cad (Cell Signaling Technology, # 14472), α-SMA (Cell Signaling Technology, #14968), TGF-β1 (Thermo Fisher Scientific, #PA1–29032), p-Smad2/3 (Cell Signaling Technology, #8828), total Smad2/3 (Cell Signaling Technology, #8685), Snail (Cell Signaling Technology, #3879) and β-actin (Cell Signaling Technology, #4970).

    Incubation:

    Article Title: Lactate Promotes Endothelial-Mesenchymal Transition via Mediating Twist1 Lactylation in Hypoxic Pulmonary Hypertension.
    Article Snippet: .. The membranes were then incubated overnight at 4 ◦C with the following specific primary antibodies: Endothelial cell markers: CD31 (1:1000, 11265-1-AP, Proteintech), VE-Cadherin (1:1000, 2500, Cell Signaling Technology, Danvers, MA, USA); Mesenchymal cell markers: α-SMA (1:2000, ab21027, Abcam), FSP1 (1:1000, ab27957, Abcam); SM22-α (1:1000, 10493-1-AP, Proteintech); Vimentin (1:1000, 5741, Cell Signaling Technology, Danvers, MA, USA); TGF-β signaling pathway-related proteins: TGF-β1 (1:1000, 10188-1-AP, proteintech), p-Smad2 (1:1000, 18338, Cell Signaling Technology), Smad2 (1:1000,12570-1-AP, proteintech), Total-Smad2/3 (1:1000, 3102, Cell Signaling Technology); Twist1 (1:1000, 25465-1-AP, Proteintech), β-actin (1:500; SC-130657, Santacruz), H3 (1:1000, 17168-1-AP, Proteintech). .. Total protein was extracted from cells or tissues using RIPA lysis buffer (P0013B, Beyotime Biotechnology) containing protease and phosphatase inhibitors (P1046, Beyotime Biotechnology) on ice.

    Article Title: Adenosine A 2A Receptor Regulates microRNA ‐181b Expression in Aorta: Therapeutic Implications for Large‐Artery Stiffness
    Article Snippet: .. Membranes were then incubated in 5% nonfat dry milk for 2 hours at room temperature with antibodies to translin and trax, which were generated in our lab, phosphorylated‐SMAD2/3 (catalog no. 8828) and total‐SMAD2/3 (catalog no. 8685) from Cell Signaling Technologies (Danvers, MA); Hsp90 (catalog no. 610419, BD Transduction Laboratories, Franklin Lakes, NJ). ..

    Article Title: Lactate Promotes Endothelial-Mesenchymal Transition via Mediating Twist1 Lactylation in Hypoxic Pulmonary Hypertension
    Article Snippet: .. The membranes were then incubated overnight at 4 °C with the following specific primary antibodies: Endothelial cell markers: CD31 (1:1000, 11265-1-AP, Proteintech), VE-Cadherin (1:1000, 2500, Cell Signaling Technology, Danvers, MA, USA); Mesenchymal cell markers: α-SMA (1:2000, ab21027, Abcam), FSP1 (1:1000, ab27957, Abcam); SM22-α (1:1000, 10493-1-AP, Proteintech); Vimentin (1:1000, 5741, Cell Signaling Technology, Danvers, MA, USA); TGF-β signaling pathway-related proteins: TGF-β1 (1:1000, 10188-1-AP, proteintech), p-Smad2 (1:1000, 18338, Cell Signaling Technology), Smad2 (1:1000,12570-1-AP, proteintech), Total-Smad2/3 (1:1000, 3102, Cell Signaling Technology); Twist1 (1:1000, 25465-1-AP, Proteintech), β-actin (1:500; SC-130657, Santacruz), H3 (1:1000, 17168-1-AP, Proteintech). .. Pan-kla (1:1000, PTM-1401, PTM BIO), CBP (1:1000, 7389, Cell Signaling Technology), p300 (1:1000, 20695-1-AP, Proteintech).

    Generated:

    Article Title: Adenosine A 2A Receptor Regulates microRNA ‐181b Expression in Aorta: Therapeutic Implications for Large‐Artery Stiffness
    Article Snippet: .. Membranes were then incubated in 5% nonfat dry milk for 2 hours at room temperature with antibodies to translin and trax, which were generated in our lab, phosphorylated‐SMAD2/3 (catalog no. 8828) and total‐SMAD2/3 (catalog no. 8685) from Cell Signaling Technologies (Danvers, MA); Hsp90 (catalog no. 610419, BD Transduction Laboratories, Franklin Lakes, NJ). ..



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    Image Search Results


    ( a ) Isolation and characterization of UMSC-EVs. ( b ) Isolation and characterization of EMSC-EVs. ( c ) Characterization of UMSC-EVs and EMSC-EVs by WB. ( d ) AA mouse model. ( e ) H&E staining of skin from AA mice. ( f ) H&E staining of skin from healthy mice. ( g ) Hair growth in each group on day 15. ( h ) Tracing results of EMSC-EVs (green fluorescence). ( i ) Venn diagram. ( j ) Volcano plot. k . Bubble plot of pathway enrichment analysis. l . miR-665 expression in UMSC-EVs and EMSC-EVs. m . Preliminary screening of miRNAs. n . Target prediction of miR-665 via miRDB database. o . Mechanism of miR-665 targeting STAT3 mRNA. p . The results of the dual-luciferase reporter assay. ( n = 3 per group; ns = not significant, *** P < 0.001, **** P < 0.0001)

    Journal: Journal of Nanobiotechnology

    Article Title: ROS-responsive hydrogel-delivered miR-665 targets STAT3 to alleviate inflammation and promote hair follicle regeneration in alopecia areata

    doi: 10.1186/s12951-026-04214-7

    Figure Lengend Snippet: ( a ) Isolation and characterization of UMSC-EVs. ( b ) Isolation and characterization of EMSC-EVs. ( c ) Characterization of UMSC-EVs and EMSC-EVs by WB. ( d ) AA mouse model. ( e ) H&E staining of skin from AA mice. ( f ) H&E staining of skin from healthy mice. ( g ) Hair growth in each group on day 15. ( h ) Tracing results of EMSC-EVs (green fluorescence). ( i ) Venn diagram. ( j ) Volcano plot. k . Bubble plot of pathway enrichment analysis. l . miR-665 expression in UMSC-EVs and EMSC-EVs. m . Preliminary screening of miRNAs. n . Target prediction of miR-665 via miRDB database. o . Mechanism of miR-665 targeting STAT3 mRNA. p . The results of the dual-luciferase reporter assay. ( n = 3 per group; ns = not significant, *** P < 0.001, **** P < 0.0001)

    Article Snippet: After transfer to polyvinylidene difluoride membranes, rabbit antibodies against phosphorylated STAT3 ( p -STAT3) (1∶2000, CST), mouse antibody against stat3 (1∶2000, CST), mouse antibody against β-actin (1∶1000, Beyotime), and mouse antibody against STAT3 (1∶1000, Beyotime) were used.

    Techniques: Isolation, Staining, Fluorescence, Expressing, Luciferase, Reporter Assay

    ( a ) STAT3 expression in lentivirus-transfected HaCaT cells and DPCs. ( b ) STAT3 expression in lentivirus-transfected HaCaT cells and DPCs before and after IFN-γ treatment. ( c ) STAT3 expression in IFN-γ-treated HaCaT cells and DPCs. ( d ) WB results showing STAT3 expression in lentivirus-transfected HaCaT cells before and after IFN-γ treatment. ( e ) WB results showing STAT3 expression in lentivirus-transfected DPCs before and after IFN-γ treatment. ( f ) Scratch assay results of HaCaT cells. ( g ) 48 h Transwell assay results of DPCs. ( h ) WB results of rescue experiments in HaCaT cells and DPCs. ( i ) Growth of hair follicles in ex vivo culture on day 5 under different treatment conditions. ( n = 3–6 per group; * P < 0.05, ** P < 0.01, *** P < 0.001)

    Journal: Journal of Nanobiotechnology

    Article Title: ROS-responsive hydrogel-delivered miR-665 targets STAT3 to alleviate inflammation and promote hair follicle regeneration in alopecia areata

    doi: 10.1186/s12951-026-04214-7

    Figure Lengend Snippet: ( a ) STAT3 expression in lentivirus-transfected HaCaT cells and DPCs. ( b ) STAT3 expression in lentivirus-transfected HaCaT cells and DPCs before and after IFN-γ treatment. ( c ) STAT3 expression in IFN-γ-treated HaCaT cells and DPCs. ( d ) WB results showing STAT3 expression in lentivirus-transfected HaCaT cells before and after IFN-γ treatment. ( e ) WB results showing STAT3 expression in lentivirus-transfected DPCs before and after IFN-γ treatment. ( f ) Scratch assay results of HaCaT cells. ( g ) 48 h Transwell assay results of DPCs. ( h ) WB results of rescue experiments in HaCaT cells and DPCs. ( i ) Growth of hair follicles in ex vivo culture on day 5 under different treatment conditions. ( n = 3–6 per group; * P < 0.05, ** P < 0.01, *** P < 0.001)

    Article Snippet: After transfer to polyvinylidene difluoride membranes, rabbit antibodies against phosphorylated STAT3 ( p -STAT3) (1∶2000, CST), mouse antibody against stat3 (1∶2000, CST), mouse antibody against β-actin (1∶1000, Beyotime), and mouse antibody against STAT3 (1∶1000, Beyotime) were used.

    Techniques: Expressing, Transfection, Wound Healing Assay, Transwell Assay, Ex Vivo